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- Title
Stable and heritable gene silencing in the malaria vector Anopheles stephensi.
- Authors
Brown, Anthony E; Bugeon, Laurence; Crisanti, Andrea; Catteruccia, Flaminia
- Abstract
Heritable RNA interference (RNAi), triggered from stably expressed transgenes with an inverted repeat (IR) configuration, is an important tool for reverse genetic studies. Here we report on the development of stable RNAi in Anopheles stephensi mosquitoes, the major vector of human malaria in Asia. Trans genic mosquitoes stably expressing a RNAi transgene, designed to produce intron-spliced double-stranded RNA (dsRNA) targeting the green fluorescent protein EGFP gene, were crossed to an EGFP-expressing target line. EGFP expression was dramatically reduced at both the protein and RNA levels. The levels of gene silencing depended upon the RNAi gene copy number and its site of integration. These results demonstrate that specific RNAi-mediated knockdown of gene function can be achieved with high efficiency in Anopheles . This will be invaluable to systematically unravel the function of Anopheles genes determining the vectorial capacity of the malaria parasite.
- Publication
Nucleic acids research, 2003, Vol 31, Issue 15, pe85
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gng085