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- Title
Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs.
- Authors
Casbon, James A; Slatter, Andrew F; Musgrave-Brown, Esther; Osborne, Robert J; Lichtenstein, Conrad P; Brenner, Sydney
- Abstract
We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
- Publication
Nucleic acids research, 2013, Vol 41, Issue 10, pe112
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkt228