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- Title
Large-scale organization of ribosomal DNA chromatin is regulated by Tip5.
- Authors
Zillner, Karina; Filarsky, Michael; Rachow, Katrin; Weinberger, Michael; Längst, Gernot; Németh, Attila
- Abstract
The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5's AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure.
- Publication
Nucleic acids research, 2013, Vol 41, Issue 10, p5251
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkt218