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- Title
Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions.
- Authors
Scheibe, Marion; Butter, Falk; Hafner, Markus; Tuschl, Thomas; Mann, Matthias
- Abstract
Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network.
- Publication
Nucleic acids research, 2012, Vol 40, Issue 19, p9897
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gks746