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- Title
Crystal structure of the UvrB dimer: insights into the nature and functioning of the UvrAB damage engagement and UvrB-DNA complexes.
- Authors
Webster, Matthew P J; Jukes, Rachael; Zamfir, Vlad S; Kay, Christopher W M; Bagnéris, Claire; Barrett, Tracey
- Abstract
UvrB has a central role in the highly conserved UvrABC pathway functioning not only as a damage recognition element but also as an essential component of the lesion tracking machinery. While it has been recently confirmed that the tracking assembly comprises a UvrA2B2 heterotetramer, the configurations of the damage engagement and UvrB-DNA handover complexes remain obscure. Here, we present the first crystal structure of a UvrB dimer whose biological significance has been verified using both chemical cross-linking and electron paramagnetic resonance spectroscopy. We demonstrate that this dimeric species stably associates with UvrA and forms a UvrA2B2-DNA complex. Our studies also illustrate how signals are transduced between the ATP and DNA binding sites to generate the helicase activity pivotal to handover and formation of the UvrB2-DNA complex, providing key insights into the configurations of these important repair intermediates.
- Publication
Nucleic acids research, 2012, Vol 40, Issue 17, p8743
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gks633