We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Direct and specific chemical control of eukaryotic translation with a synthetic RNA-protein interaction.
- Authors
Goldfless, Stephen J; Belmont, Brian J; de Paz, Alexandra M; Liu, Jessica F; Niles, Jacquin C
- Abstract
Sequence-specific RNA-protein interactions, though commonly used in biological systems to regulate translation, are challenging to selectively modulate. Here, we demonstrate the use of a chemically-inducible RNA-protein interaction to regulate eukaryotic translation. By genetically encoding Tet Repressor protein (TetR)-binding RNA elements into the 5'-untranslated region (5'-UTR) of an mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5'-UTR contexts, this system efficiently regulates the expression of multiple target genes, and is sufficiently stringent to distinguish functional from non-functional RNA-TetR interactions. Using a reverse TetR variant, we illustrate the potential for expanding the regulatory properties of the system through protein engineering strategies.
- Publication
Nucleic acids research, 2012, Vol 40, Issue 9, pe64
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gks028