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- Title
Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine.
- Authors
Szwagierczak, Aleksandra; Brachmann, Andreas; Schmidt, Christine S; Bultmann, Sebastian; Leonhardt, Heinrich; Spada, Fabio
- Abstract
In mammalian genomes a sixth base, 5-hydroxymethylcytosine ((hm)C), is generated by enzymatic oxidation of 5-methylcytosine ((m)C). This discovery has raised fundamental questions about the functional relevance of (hm)C in mammalian genomes. Due to their very similar chemical structure, discrimination of the rare (hm)C against the far more abundant (m)C is technically challenging and to date no methods for direct sequencing of (hm)C have been reported. Here, we report on a purified recombinant endonuclease, PvuRts1I, which selectively cleaves (hm)C-containing sequences. We determined the consensus cleavage site of PvuRts1I as (hm)CN(11-12)/N(9-10)G and show first data on its potential to interrogate (hm)C patterns in mammalian genomes.
- Publication
Nucleic acids research, 2011, Vol 39, Issue 12, p5149
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkr118