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- Title
Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA.
- Authors
Szwagierczak, Aleksandra; Bultmann, Sebastian; Schmidt, Christine S; Spada, Fabio; Leonhardt, Heinrich
- Abstract
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
- Publication
Nucleic acids research, 2010, Vol 38, Issue 19, pe181
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkq684