We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A flexible and efficient template format for circular consensus sequencing and SNP detection.
- Authors
Travers, Kevin J; Chin, Chen-Shan; Rank, David R; Eid, John S; Turner, Stephen W
- Abstract
A novel template design for single-molecule sequencing is introduced, a structure we refer to as a SMRTbell template. This structure consists of a double-stranded portion, containing the insert of interest, and a single-stranded hairpin loop on either end, which provides a site for primer binding. Structurally, this format resembles a linear double-stranded molecule, and yet it is topologically circular. When placed into a single-molecule sequencing reaction, the SMRTbell template format enables a consensus sequence to be obtained from multiple passes on a single molecule. Furthermore, this consensus sequence is obtained from both the sense and antisense strands of the insert region. In this article, we present a universal method for constructing these templates, as well as an application of their use. We demonstrate the generation of high-quality consensus accuracy from single molecules, as well as the use of SMRTbell templates in the identification of rare sequence variants.
- Publication
Nucleic acids research, 2010, Vol 38, Issue 15, pe159
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkq543