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- Title
Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations.
- Authors
Petrov, Alexey; Puglisi, Joseph D
- Abstract
Site-specific labeling of Escherichia coli ribosomes has allowed application of single-molecule fluorescence spectroscopy and force methods to probe the mechanism of translation. To apply these approaches to eukaryotic translation, eukaryotic ribosomes must be specifically labeled with fluorescent labels and molecular handles. Here, we describe preparation and labeling of the small and large yeast ribosomal subunits. Phylogenetically variable hairpin loops in ribosomal RNA are mutated to allow hybridization of oligonucleotides to mutant ribosomes. We demonstrate specific labeling of the ribosomal subunits, and their use in single-molecule fluorescence and force experiments.
- Publication
Nucleic acids research, 2010, Vol 38, Issue 13, pe143
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkq390