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- Title
RosettaBackrub--a web server for flexible backbone protein structure modeling and design.
- Authors
Lauck, Florian; Smith, Colin A; Friedland, Gregory F; Humphris, Elisabeth L; Kortemme, Tanja
- Abstract
The RosettaBackrub server (http://kortemmelab.ucsf.edu/backrub) implements the Backrub method, derived from observations of alternative conformations in high-resolution protein crystal structures, for flexible backbone protein modeling. Backrub modeling is applied to three related applications using the Rosetta program for structure prediction and design: (I) modeling of structures of point mutations, (II) generating protein conformational ensembles and designing sequences consistent with these conformations and (III) predicting tolerated sequences at protein-protein interfaces. The three protocols have been validated on experimental data. Starting from a user-provided single input protein structure in PDB format, the server generates near-native conformational ensembles. The predicted conformations and sequences can be used for different applications, such as to guide mutagenesis experiments, for ensemble-docking approaches or to generate sequence libraries for protein design.
- Publication
Nucleic acids research, 2010, Vol 38, Issue Web Server issue, pW569
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkq369