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- Title
FtsK translocation on DNA stops at XerCD-dif.
- Authors
Graham, James E; Sivanathan, Viknesh; Sherratt, David J; Arciszewska, Lidia K
- Abstract
Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes.
- Publication
Nucleic acids research, 2010, Vol 38, Issue 1, p72
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkp843