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- Title
Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.
- Authors
Qiu, Caihong; Ma, Yinghong; Wang, Jianquan; Peng, Shuping; Huang, Yingqun
- Abstract
Lin28 acts as a repressor of microRNA processing and as a post-transcriptional regulatory factor for a subset of mRNAs. Here we report that in human embryonic stem cells Lin28 facilitates the expression of the pivotal pluripotency factor Oct4 at the post-transcriptional level. We provide evidence that Lin28 binds Oct4 mRNA directly through high affinity sites within its coding region and that an interaction between Lin28 and RNA helicase A (RHA) may play a part in the observed regulation. We further demonstrate that decreasing RHA levels impairs Lin28-dependent stimulation of translation in a reporter system. Taken together with previous studies showing that RHA is required for efficient translation of a specific class of mRNAs, these findings suggest a novel mechanism by which Lin28 may affect target mRNA expression and represent the first evidence of post-transcriptional regulation of Oct4 expression by Lin28 in human embryonic stem cells.
- Publication
Nucleic acids research, 2010, Vol 38, Issue 4, p1240
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkp1071