We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli.
- Authors
Neupert, Juliane; Karcher, Daniel; Bock, Ralph
- Abstract
RNA thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts. Here, we report the de novo design of considerably simpler RNA thermometers that provide useful RNA-only tools to regulate bacterial gene expression by a shift in the growth temperature. We show that a single small stem-loop structure containing the ribosome binding site is sufficient to construct synthetic RNA thermometers that work efficiently at physiological temperatures. Our data suggest that the thermometers function by a simple melting mechanism and thus provide minimum size on/off switches to experimentally induce or repress gene expression by temperature.
- Publication
Nucleic acids research, 2008, Vol 36, Issue 19, pe124
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkn545