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- Title
Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element.
- Authors
Schwalbe, Martin; Ohlenschläger, Oliver; Marchanka, Aliaksandr; Ramachandran, Ramadurai; Häfner, Sabine; Heise, Tilman; Görlach, Matthias
- Abstract
Chronic hepatitis B virus (HBV) infections may lead to severe diseases like liver cirrhosis or hepatocellular carcinoma (HCC). The HBV post-transcriptional regulatory element (HPRE) facilitates the nuclear export of unspliced viral mRNAs, contains a splicing regulatory element and resides in the 3'-region of all viral transcripts. The HPRE consists of three sub-elements alpha (nucleotides 1151-1346), beta1 (nucleotides 1347-1457) and beta2 (nucleotides 1458-1582), which confer together full export competence. Here, we present the NMR solution structure (pdb 2JYM) of the stem-loop alpha (SLalpha, nucleotides 1292-1321) located in the sub-element alpha. The SLalpha contains a CAGGC pentaloop highly conserved in hepatoviruses, which essentially adopts a CUNG-like tetraloop conformation. Furthermore, the SLalpha harbours a single bulged G residue flanked by A-helical regions. The structure is highly suggestive of serving two functions in the context of export of unspliced viral RNA: binding sterile alpha motif (SAM-) domain containing proteins and/or preventing the utilization of a 3'-splice site contained within SLalpha.
- Publication
Nucleic acids research, 2008, Vol 36, Issue 5, p1681
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkn006