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- Title
Targeted high-throughput sequencing of tagged nucleic acid samples.
- Authors
Meyer, Matthias; Stenzel, Udo; Myles, Sean; Prüfer, Kay; Hofreiter, Michael
- Abstract
High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.
- Publication
Nucleic acids research, 2007, Vol 35, Issue 15, pe97
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkm566