We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.
- Authors
Grissa, Ibtissem; Vergnaud, Gilles; Pourcel, Christine
- Abstract
Clustered regularly interspaced short palindromic repeats (CRISPRs) constitute a particular family of tandem repeats found in a wide range of prokaryotic genomes (half of eubacteria and almost all archaea). They consist of a succession of highly conserved regions (DR) varying in size from 23 to 47 bp, separated by similarly sized unique sequences (spacer) of usually viral origin. A CRISPR cluster is flanked on one side by an AT-rich sequence called the leader and assumed to be a transcriptional promoter. Recent studies suggest that this structure represents a putative RNA-interference-based immune system. Here we describe CRISPRFinder, a web service offering tools to (i) detect CRISPRs including the shortest ones (one or two motifs); (ii) define DRs and extract spacers; (iii) get the flanking sequences to determine the leader; (iv) blast spacers against Genbank database and (v) check if the DR is found elsewhere in prokaryotic sequenced genomes. CRISPRFinder is freely accessible at http://crispr.u-psud.fr/Server/CRISPRfinder.php.
- Publication
Nucleic acids research, 2007, Vol 35, Issue Web Server issue, pW52
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkm360