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- Title
CTD kinase I is involved in RNA polymerase I transcription.
- Authors
Bouchoux, Céline; Hautbergue, Guillaume; Grenetier, Sabrina; Carles, Christophe; Riva, Michel; Goguel, Valérie
- Abstract
RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA synthesis at the level of transcription elongation, pre-mRNA 3' formation and nuclear export. Here, we report that CTDK-I is also involved in ribosomal RNA synthesis. We show that CTDK-I is localized in part in the nucleolus. In its absence, nucleolar structure and RNA polymerase I transcription are affected. In vitro experiments show an impairment of the Pol I transcription machinery. Remarkably, RNA polymerase I co-precipitates from cellular extracts with Ctk1, the kinase subunit of the CTDK-I complex. In vitro analysis further demonstrates a direct interaction between RNA polymerase I and Ctk1. The results suggest that CTDK-I might participate in the regulation of distinct nuclear transcriptional machineries, thus playing a role in the adaptation of the global transcriptional response to growth signalling.
- Publication
Nucleic acids research, 2004, Vol 32, Issue 19, p5851
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkh927