We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A dynamic, web-accessible resource to process raw microarray scan data into consolidated gene expression values: importance of replication.
- Authors
Le Meur, Nolwenn; Lamirault, Guillaume; Bihouée, Audrey; Steenman, Marja; Bédrine-Ferran, Hélène; Teusan, Raluca; Ramstein, Gérard; Léger, Jean J
- Abstract
We propose a freely accessible web-based pipeline, which processes raw microarray scan data to obtain experimentally consolidated gene expression values. The tool MADSCAN, which stands for MicroArray Data Suites of Computed ANalysis, makes a practical choice among the numerous methods available for filtering, normalizing and scaling of raw microarray expression data in a dynamic and automatic way. Different statistical methods have been adapted to extract reliable information from replicate gene spots as well as from replicate microarrays for each biological situation under study. A carefully constructed experimental design thus allows to detect outlying expression values and to identify statistically significant expression values, together with a list of quality controls with proposed threshold values. The integrated processing procedure described here, based on multiple measurements per gene, is decisive for reliably monitoring subtle gene expression changes typical for most biological events.
- Publication
Nucleic acids research, 2004, Vol 32, Issue 18, p5349
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkh870