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- Title
Identification of stop codon readthrough genes in Saccharomyces cerevisiae.
- Authors
Namy, Olivier; Duchateau-Nguyen, Guillemette; Hatin, Isabelle; Hermann-Le Denmat, Sylvie; Termier, Michel; Rousset, Jean-Pierre
- Abstract
We specifically sought genes within the yeast genome controlled by a non-conventional translation mechanism involving the stop codon. For this reason, we designed a computer program using the yeast database genomic regions, and seeking two adjacent open reading frames separated only by a unique stop codon (called SORFs). Among the 58 SORFs identified, eight displayed a stop codon bypass level ranging from 3 to 25%. For each of the eight sequences, we demonstrated the presence of a poly(A) mRNA. Using isogenic [PSI(+)] and [psi(-)] yeast strains, we showed that for two of the sequences the mechanism used is a bona fide readthrough. However, the six remaining sequences were not sensitive to the PSI state, indicating either a translation termination process independent of eRF3 or a new stop codon bypass mechanism. Our results demonstrate that the presence of a stop codon in a large ORF may not always correspond to a sequencing error, or a pseudogene, but can be a recoding signal in a functional gene. This emphasizes that genome annotation should take into account the fact that recoding signals could be more frequently used than previously expected.
- Publication
Nucleic acids research, 2003, Vol 31, Issue 9, p2289
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkg330