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- Title
The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells.
- Authors
Ogilvie, V C; Wilson, B J; Nicol, S M; Morrice, N A; Saunders, L R; Barber, G N; Fuller-Pace, F V
- Abstract
The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as hetero-dimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from approximately 150 to approximately 400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.
- Publication
Nucleic acids research, 2003, Vol 31, Issue 5, p1470
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/gkg236