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- Title
RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA).
- Authors
Verbeure, B; Lescrinier, E; Wang, J; Herdewijn, P
- Abstract
Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.
- Publication
Nucleic acids research, 2001, Vol 29, Issue 24, p4941
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/29.24.4941