We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A second NAD(+)-dependent DNA ligase (LigB) in Escherichia coli.
- Authors
Sriskanda, V; Shuman, S
- Abstract
Escherichia coli DNA ligase (LigA) is the prototype of the NAD(+)-dependent class of DNA ligases found in all bacteria. Here we report the characterization of E.coli LigB, a second NAD(+)-dependent DNA ligase identified by virtue of its sequence similarity to LigA. LigB differs from LigA in that it lacks the BRCA1 C-terminus domain (BRCT) and two of the four Zn-binding cysteines that are present in LigA and all other bacterial NAD(+) ligases. We found that recombinant LigB catalyzed strand joining on a singly-nicked DNA in the presence of a divalent cation and NAD(+), and that LigB reacted with NAD(+) to form a covalent ligase-adenylate intermediate. Alanine substitution for the motif I lysine ((126)KxDG) abolished nick joining and ligase-adenylate formation by LigB, thus confirming that the ligase and adenylyltransferase activities are intrinsic to the LigB protein.
- Publication
Nucleic acids research, 2001, Vol 29, Issue 24, p4930
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/29.24.4930