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- Title
Structural and mechanistic conservation in DNA ligases.
- Authors
Doherty, A J; Suh, S W
- Abstract
DNA ligases are enzymes required for the repair, replication and recombination of DNA. DNA ligases catalyse the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA. Despite their occurrence in all organisms, DNA ligases show a wide diversity of amino acid sequences, molecular sizes and properties. The enzymes fall into two groups based on their cofactor specificity, those requiring NAD(+) for activity and those requiring ATP. The eukaryotic, viral and archael bacteria encoded enzymes all require ATP. NAD(+)-requiring DNA ligases have only been found in prokaryotic organisms. Recently, the crystal structures of a number of DNA ligases have been reported. It is the purpose of this review to summarise the current knowledge of the structure and catalytic mechanism of DNA ligases.
- Publication
Nucleic acids research, 2000, Vol 28, Issue 21, p4051
- ISSN
1362-4962
- Publication type
Review
- DOI
10.1093/nar/28.21.4051