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- Title
DNA repair in a yeast origin of replication: contributions of photolyase and nucleotide excision repair.
- Authors
Suter, B; Wellinger, R E; Thoma, F
- Abstract
DNA damage formation and repair are tightly linked to protein-DNA interactions in chromatin. We have used minichromosomes in yeast as chromatin substrates in vivo to investigate how nucleotide excision repair (NER) and repair by DNA-photolyase (photoreactivation) remove pyrimidine dimers from an origin of replication ( ARS1 ). The ARS1 region is nuclease sensitive and flanked by nucleosomes on both sides. Photoreactivation was generally faster than NER at all sites. Site-specific heterogeneity of repair was observed for both pathways. This heterogeneity was different for NER and photoreactivation and it was altered in a minichromosome where ARS1 was transcribed. The results indicate distinct inter-actions of the repair systems with protein complexes bound in the ARS region (ORC, Abf1) and a predominant role of photolyase in CPD repair of an origin of replication.
- Publication
Nucleic acids research, 2000, Vol 28, Issue 10, p2060
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/28.10.2060