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- Title
Amplification of cDNA ends based on template-switching effect and step-out PCR.
- Authors
Matz, M; Shagin, D; Bogdanova, E; Britanova, O; Lukyanov, S; Diatchenko, L; Chenchik, A
- Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.
- Publication
Nucleic acids research, 1999, Vol 27, Issue 6, p1558
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/27.6.1558