We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.
- Authors
Piechaczek, C; Fetzer, C; Baiker, A; Bode, J; Lipps, H J
- Abstract
We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.
- Publication
Nucleic acids research, 1999, Vol 27, Issue 2, p426
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/27.2.426