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- Title
Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags.
- Authors
van den Berg, A; van der Leij, J; Poppema, S
- Abstract
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene expression (SAGE) approach is how to further analyse the unknown tags. We have developed an RT-PCR-based method, rapid analysis of unknown SAGE tags (RAST-PCR), to analyse the expression of the corresponding genes. This approach can be used as a screening method to investigate whether or not the gene is differentially expressed between several cell types of interest.
- Publication
Nucleic acids research, 1999, Vol 27, Issue 17, pe17
- ISSN
1362-4962
- Publication type
Journal Article
- DOI
10.1093/nar/27.17.e17