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- Title
Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction.
- Authors
Falchetti, M L; Levi, A; Molinari, P; Verna, R; D'Ambrosio, E
- Abstract
Telomeric Repeat Amplification Protocol (TRAP) is a sensitive procedure to measure telomerase activity in small samples of cell or tissue extracts. Due to the strict correlation between high levels of telomerase activity and neoplastic transformation, TRAP assay could provide an important diagnostic marker of malignancy. Although the original TRAP assay is very sensitive and some improvements have been described, occasional artifacts still persist in the modified procedures. Here we describe how changes in the sequence of the primer used for the amplification step enhance the reproducibility and sensitivity in the TRAP assay.
- Publication
Nucleic acids research, 1998, Vol 26, Issue 3, p862
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/26.3.862