We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A rapid and sensitive assay for histone acetyl-transferase activity.
- Authors
Ait-Si-Ali, S; Ramirez, S; Robin, P; Trouche, D; Harel-Bellan, A
- Abstract
Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.
- Publication
Nucleic acids research, 1998, Vol 26, Issue 16, p3869
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/26.16.3869