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- Title
Two color hybridization analysis using high density oligonucleotide arrays and energy transfer dyes.
- Authors
Hacia, J G; Edgemon, K; Sun, B; Stern, D; Fodor, S P; Collins, F S
- Abstract
High density oligonucleotide arrays (DNA chips) have been used in two color mutational analysis of the 3.43 kb exon 11 of the hereditary breast and ovarian cancer gene BRCA1 . Two color analysis allows competitive hybridization between a reference standard and an unknown sample, improving the performance of the assay. Fluorescein and phycoerythrin dyes werepreviously used due to their compatibility with a single line 488 nm excitation source. Here we show that an alternative dye combination, containing the energy transfer dye system phycoerythrin*cy5 along with phycoerythrin, provides more evenly matched signal intensities and decreased spectral overlap between the two fluorophores, while maintaining compatibility with a 488 nm excitation source.
- Publication
Nucleic acids research, 1998, Vol 26, Issue 16, p3865
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/26.16.3865