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- Title
Recombination-mediated PCR-directed plasmid construction in vivo in yeast.
- Authors
Oldenburg, K R; Vo, K T; Michaelis, S; Paddon, C
- Abstract
We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.
- Publication
Nucleic acids research, 1997, Vol 25, Issue 2, p451
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/25.2.451