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- Title
Betaine improves the PCR amplification of GC-rich DNA sequences.
- Authors
Henke, W; Herdel, K; Jung, K; Schnorr, D; Loening, S A
- Abstract
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.
- Publication
Nucleic acids research, 1997, Vol 25, Issue 19, p3957
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/25.19.3957