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- Title
Nanoliter scale PCR with TaqMan detection.
- Authors
Kalinina, O; Lebedeva, I; Brown, J; Silver, J
- Abstract
We monitored PCR in volumes of the order of 10 nl in glass microcapillaries using a fluorescence energy transfer assay in which fluorescence increases if product is made due to template-dependent nucleolytic degradation of an internally quenched probe (TaqMan assay). This assay detected single starting template molecules in dilutions of genomic DNA. The results suggest that it may be feasible to determine the number of template molecules in a sample by counting the number of positive PCRs in a set of replicate reactions using terminally diluted sample. Since the assay system is closed and potentially automatable, it has promise for clinical applications.
- Publication
Nucleic acids research, 1997, Vol 25, Issue 10, p1999
- ISSN
0305-1048
- Publication type
Journal Article
- DOI
10.1093/nar/25.10.1999