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- Title
Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006.
- Authors
Wolfgang Witte; Birgit Strommenger; Christa Cuny; Dagmar Heuck; Ulrich Nuebel
- Abstract
Objectives The aim of this paper is to attribute Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) to clonal lineages by molecular typing with special reference to isolates exhibiting spa type t008/multilocus sequence type (MLST) ST8 [widely disseminated in the USA as âcommunity-associated MRSA (caMRSA) USA300â]. Methods PVL-positive MRSA (n = 117) were detected among 4815 MRSA sent to the German National Reference Laboratory for typing. These isolates were analysed by PFGE, spa typing, multilocus sequence typing, grouping of SCCmec elements and PCR detection of arcA, msr(A), mph(B) and the â¥6 AT repeat unit in the SACOL0058 sequence. Results Among the 117 isolates, 80 exhibited spa type t044 (corresponding to MLST ST80) and 23 exhibited spa type t008/MLST ST8. Other spa types were sporadically represented. Further characterization of isolates exhibiting t008/ST8 by PCR [arcA, msr(A), mph(B), â¥6 AT repeat signature] indicates the arrival of caMRSA âUSA300â in Central Europe. Conclusions caMRSA ST80 still predominate; however, caMRSA ST8 exhibiting the characteristics of the âUSA300â clone became the second most frequent. Routine detection of this clone in clinical bacteriology can be easily performed by PCR.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2007, Vol 60, Issue 6, p1258
- ISSN
0305-7453
- Publication type
Academic Journal
- DOI
10.1093/jac/dkm384