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- Title
Predominance and genetic diversity of community- and hospital-acquired CTX-M extended-spectrum β-lactamases in York, UK.
- Authors
C. J. Munday; G. M. Whitehead; N. J. Todd; M. Campbell; P. M. Hawkey
- Abstract
Objectives: This study was conducted to detect the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae within the faecal flora of both community- and hospital-based patients in York and to characterize the blaTEM, blaSHV and blaCTX-M genes present in these isolates.Methods: One thousand faeces samples were collected and screened at York Hospital during October–December 2003. Ninety-five non-duplicate Enterobacteriaceae isolates resistant to third-generation cephalosporins were recovered; 22 isolates were selected for further study on the basis of a positive double disc diffusion test for ESBL production. Antibiotic susceptibility testing was performed to a range of antibiotics. The TEM, SHV and CTX-M genes were detected by PCR and the DNA sequenced.Results: The distribution of ESBL-positive isolates from the hospital and community was 1.4:1. These included nine Escherichia coli, seven Enterobacter cloacae, four Citrobacter freundii and a single isolate each of Klebsiella spp. and Salmonella spp. A total of 17 isolates contained blaCTX-M (five blaCTX-M-15, three blaCTX-M-14 and nine blaCTX-M-9). ISEcp1 was present in isolates expressing CTX-M-14 and -15, but was absent upstream of In60-associated blaCTX-M-9. E. coli isolates also contained either a blaTEM-1 or blaTEM-2, whereas six of the E. cloacae carried blaSHV-12 and the Klebsiella spp. blaSHV-36 in addition to blaCTX-M-9. The single Salmonella spp. carried blaSHV-12.Conclusions: The overall prevalence of ESBL in isolates of Enterobacteriaceae from York was 1.9%. ESBL-producing isolates were found in both the community and hospital, with the CTX-M type most common. This is also the first report of an ESBL-producing Salmonella in the UK.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2004, Vol 54, Issue 3, p628
- ISSN
0305-7453
- Publication type
Academic Journal
- DOI
10.1093/jac/dkh397