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- Title
Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112).
- Authors
Tahara-Hanaoka, Satoko; Shibuya, Kazuko; Onoda, Yuko; Zhang, Hua; Yamazaki, Satoshi; Miyamoto, Akitomo; Honda, Shin-Ichiro; Lanier, Lewis L; Shibuya, Akira
- Abstract
CD226 (DNAM-1) is an adhesion molecule involved in NK and T cell-mediated cytotoxicity against certain tumors. Here, we have identified the human poliovirus receptor-related (PRR) family members CD155 [poliovirus receptor (PVR)] and CD112 (nectin-2/PRR-2) as the ligands for human CD226. Ectopic expression of human CD155 and/or CD112 rendered mouse BW5147 T cells more susceptible to IL-2-activated T and NK cell-mediated cytotoxicity, and killing was specifically inhibited by anti-CD226 mAb, demonstrating functional interactions of CD226 with CD155 and CD112. Although the binding affinities between soluble CD226 and CD155 or CD112 were comparable, the homophilic interaction of cell-surface CD112 may adversely affect CD226 binding to CD112. We also demonstrate that ligation of CD226 and LFA-1 with their respective ligands cooperates in triggering cytotoxicity and cytokine secretion by T and NK cells.
- Publication
International immunology, 2004, Vol 16, Issue 4, p533
- ISSN
0953-8178
- Publication type
Journal Article
- DOI
10.1093/intimm/dxh059