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- Title
Stimulation of human cytotoxic T cells with HIV-1-derived peptides presented by recombinant HLA-A2 peptide complexes.
- Authors
Walter, J B; Brander, C; Mammen, M; Garboczi, D N; Kalams, S A; Whitesides, G M; Walker, B D; Eisen, H N
- Abstract
HLA-A2 heavy chain and beta 2-microglobulin were expressed in Escherichia coli, and refolded in the presence of peptides derived from HIV-1 RT and gag proteins. When recombinant HLA-A2 molecules were attached to cells lacking HLA-A2, the cells became susceptible to lysis by HLA-A2-restricted cytotoxic T lymphocyte (CTL) clones specific for peptides derived from RT and gag proteins. Limiting dilution analyses of peripheral blood mononuclear cells from HIV-1-infected individuals showed that the recombinant HLA-A2 peptide complexes covalently immobilized on microspheres stimulated the development of HLA-A2 peptide-specific CTL. Preformed HLA-peptide complexes may provide an alternative to immunization procedures that depend upon intracellular processing of antigen to elicit T cell responses.
- Publication
International immunology, 1997, Vol 9, Issue 3, p451
- ISSN
0953-8178
- Publication type
Journal Article
- DOI
10.1093/intimm/9.3.451