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- Title
ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.
- Authors
Li, Xiangyun; Krawetz, Roman; Liu, Shiying; Meng, Guoliang; Rancourt, Derrick E
- Abstract
Efficient slow freezing protocols within serum-free and feeder-free culture systems are crucial for the clinical application of human embryonic stem (hES) cells. Frequently, however, hES cells must be cryopreserved as clumps when using conventional slow freezing protocols, leading to lower survival rates during freeze-thaw and limiting their recovery and growth efficiency after thawing, as well as limiting downstream applications that require single cell suspensions. We describe a novel method to increase freeze-thaw survival and proliferation rate of single hES cells in serum-free and feeder-free culture conditions.
- Publication
Human reproduction (Oxford, England), 2009, Vol 24, Issue 3, p580
- ISSN
1460-2350
- Publication type
Journal Article
- DOI
10.1093/humrep/den404