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- Title
Distinct GAGE and MAGE-A expression during early human development indicate specific roles in lineage differentiation.
- Authors
Morten F. Gjerstorff; Linda Harkness; Moustapha Kassem; Ulrik Frandsen; Ole Nielsen; Melissa Lutterodt; Kjeld Møllgård; Henrik J. Ditzel
- Abstract
BACKGROUND Expression of cancer/testis-associated proteins (CTAs) has traditionally been considered to be restricted to germ cells in normal tissues and to different types of malignancies. We have evaluated the potential role of CTAs in early human development. METHODS Using immunohistochemistry and RT–PCR, we investigated the expression of CTAs in differentiated human embryonic stem cells (hESC) and in late embryos and early fetuses. RESULTS We found that melanoma antigen A (MAGE-A) family members were expressed during differentiation of hESC to embryoid bodies and in teratomas, and overlapped with expression of the neuroectodermal markers beta-tubulin 3, Pax6 and nestin. A widespread expression of MAGE-A was also observed in neurons of the early developing central nervous system and peripheral nerves. G antigen (GAGE) expression was present in the early ectoderm of embryos, including cells of the ectodermal ring and apical epidermal ridge. Neuroectodermal cells in the floor plate and adjacent processes and endfeet of radial glial cells also expressed GAGE. In addition, GAGE family members were expressed in the peripheral adrenal cortex of 6–9-week-old embryos and fetuses, which specifically correlated with massive cellular proliferation and establishment of the definitive and fetal zones. Overlapping expression of MAGE-A and GAGE proteins occurred in migrating primordial germ cells. CONCLUSIONS Our results show that CTAs, in addition to their role in germ cells, may be involved in early development of various types of somatic cells, and suggest that they are implicated in specific differentiation processes.
- Publication
Human Reproduction, 2008, Vol 23, Issue 10, p2194
- ISSN
0268-1161
- Publication type
Academic Journal
- DOI
10.1093/humrep/den262