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- Title
A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells.
- Authors
Hovatta, Outi; Mikkola, Milla; Gertow, Karin; Strömberg, Anne-Marie; Inzunza, José; Hreinsson, Julius; Rozell, Björn; Blennow, Elisabeth; Andäng, Michael; Ahrlund-Richter, Lars
- Abstract
Human embryonic stem (hES) cell lines were first cultured using fetal mouse fibroblasts as feeder cells. To avoid feeders and to reduce the amount of xeno-components, Matrigel- and laminin-coated dishes, and conditioned mouse feeder cell medium have been used, and hES cells have also been cultured on human fetal muscle and skin, and adult Fallopian tube epithelial cells.
- Publication
Human reproduction (Oxford, England), 2003, Vol 18, Issue 7, p1404
- ISSN
0268-1161
- Publication type
Journal Article
- DOI
10.1093/humrep/deg290