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- Title
Hyper-expression of human apolipoprotein E4 in astroglia and neurons does not enhance amyloid deposition in transgenic mice.
- Authors
Lesuisse, C; Xu, G; Anderson, J; Wong, M; Jankowsky, J; Holtz, G; Gonzalez, V; Wong, P C; Price, D L; Tang, F; Wagner, S; Borchelt, D R
- Abstract
Recent studies in mice have clearly demonstrated that eliminating Apo E alters the rate, character and distribution of A beta deposits. In the present study, we asked whether elevating the levels of Apo E can, in a dominant fashion, influence amyloid deposition. We expressed human (Hu) Apo E4 via the mouse prion protein promoter, resulting in high expression in both astrocytes and neurons; only astrocytes efficiently secreted Hu Apo E4 (at least 5-fold more than endogenous). Mice hyper-expressing Hu Apo E4 developed normally and lived normal lifespans. The co-expression of Hu Apo E4 with a mutant amyloid precursor protein (APP) (Mo/Hu APPswe) or mutant APP and mutant presenilin (PS1dE9) did not lead to proportional changes in the age of appearance, relative burden, character or distribution of A beta deposits. We suggest that these data are best explained by proposing that the mechanisms by which Apo E influences A beta deposition involves an aspect of its normal function that is not augmented by hyper-expression.
- Publication
Human molecular genetics, 2001, Vol 10, Issue 22, p2525
- ISSN
0964-6906
- Publication type
Journal Article
- DOI
10.1093/hmg/10.22.2525