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- Title
Neural tube defects and neuroepithelial cell death in Tulp3 knockout mice.
- Authors
Ikeda, A; Ikeda, S; Gridley, T; Nishina, P M; Naggert, J K
- Abstract
The tubby-like protein 3 (Tulp3) gene has been identified as a member of a small novel gene family which is primarily neuronally expressed. Mutations in two of the family members, tub and tulp1, have been shown to cause neurosensory disorders. To determine the in vivo function of Tulp3, we have generated a germline mutation in the mouse Tulp3 gene by homologous recombination. Embryos homozygous for the Tulp3 mutant allele exhibit failure of neural tube closure, and die by embryonic day 14.5. Failure of cranial neural tube closure coincided with increased neuroepithelial apoptosis specifically in the hindbrain and the caudal neural tube. In addition, the number of betaIII-tubulin positive cells is significantly decreased in the hindbrain of Tulp3(-/-) embryos. These results suggest that disruption of the Tulp3 gene affects the development of a neuronal cell population. Interestingly, some Tulp3 heterozygotes also manifest embryonic lethality with neuroepithelial cell death. Our results demonstrate that the Tulp3 gene is essential for embryonic development in mice.
- Publication
Human molecular genetics, 2001, Vol 10, Issue 12, p1325
- ISSN
0964-6906
- Publication type
Journal Article
- DOI
10.1093/hmg/10.12.1325