We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A new kind of carbohydrate array, its use for profiling antiglycan antibodies, and the discovery of a novel human cellulose-binding antibody.
- Authors
Schwarz, Mikael; Spector, Larissa; Gargir, Ari; Shtevi, Avraham; Gortler, Monica; Altstock, Rom T; Dukler, Avinoam A; Dotan, Nir
- Abstract
In this study, we use a novel glycan array to analyze the glycan-binding antibody repertoire in a pool of affinity-purified IgG collected from a healthy human population. The glycan array used is based on mono- and oligosaccharides covalently linked to the surface via a long linker at their reducing ends. They are thus presented to the medium with a well-defined orientation and are accessible for specific binding by glycan-binding proteins, such as antibodies and lectins. A novel anticellulose antibody was detected that binds specifically to beta4-linked saccharides with a preference for glucopyranose over galactopyranose residues. We also found previously known antiglycan antibodies against mono- and oligosaccharides that are constituents of commonly occurring bacterial polysaccharides. We propose that this array can facilitate high-throughput screening of glycan-binding proteins and the search for biomarkers for personalized medicine.
- Publication
Glycobiology, 2003, Vol 13, Issue 11, p749
- ISSN
0959-6658
- Publication type
Journal Article
- DOI
10.1093/glycob/cwg091