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- Title
Pathways change in expression during replicative aging in Saccharomyces cerevisiae.
- Authors
Yiu, Gloria; McCord, Alejandra; Wise, Alison; Jindal, Rishi; Hardee, Jennifer; Kuo, Allen; Shimogawa, Michelle Yuen; Cahoon, Laty; Wu, Michelle; Kloke, John; Hardin, Johanna; Mays Hoopes, Laura L
- Abstract
Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
- Publication
The journals of gerontology. Series A, Biological sciences and medical sciences, 2008, Vol 63, Issue 1, p21
- ISSN
1079-5006
- Publication type
Journal Article
- DOI
10.1093/gerona/63.1.21