We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat.
- Authors
Li Huang; Brooks, Steven A.; Wanlong Li; Fellers, John P.; Trick, Harold N.; Gill, Bikrain S.
- Abstract
We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped lo a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 lochs detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.
- Publication
Genetics, 2003, Vol 164, Issue 2, p655
- ISSN
0016-6731
- Publication type
Academic Journal
- DOI
10.1093/genetics/164.2.655