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- Title
The role of the carboxyterminal domain of RNA polymerase II in regulating origins of DNA replication in Saccharomyces cerevisiae.
- Authors
Gauthier, Laura; Dziak, Renata; Kramer, David J H; Leishman, David; Song, Xiaomin; Ho, Jason; Radovic, Maja; Bentley, David; Yankulov, Krassimir
- Abstract
MCM (minichromosome maintenance) proteins function as a replication licensing factor (RLF-M), which contributes to limiting initiation of DNA replication to once per cell cycle. In the present study we show that a truncation of the pol II CTD in a S. cerevisiae strain harboring a mutation in mcm5 partially reverses its ts phenotype and improves maintenance of CEN/ARS minichromosomes. We correlate this phenotype to effects on DNA replication rather than to effects on transcription or specific gene expression. We also demonstrate that a similar truncation of the CTD reduces minichromosome stability and impairs stimulation of DNA replication by trans-activators and that tethering of recombinant pol II CTD to an origin of replication has a significant stimulatory effect on minichromosome stability. Furthermore, we show that pol II is recruited to ARS1. We propose that in S. cerevisiae a mechanism of coordinating pol II transcription and DNA replication is mediated by the CTD of pol II.
- Publication
Genetics, 2002, Vol 162, Issue 3, p1117
- ISSN
0016-6731
- Publication type
Journal Article
- DOI
10.1093/genetics/162.3.1117