We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.
- Authors
Chan, C S; Botstein, D
- Abstract
We have developed a colony papillation assay for monitoring the copy number of genetically marked chromosomes II and III in Saccharomyces cerevisiae. The unique feature of this assay is that it allows detection of a gain of the marked chromosomes even if there is a gain of the entire set of chromosomes (increase-in-ploidy). This assay was used to screen for chromosome-gain or increase-in-ploidy mutants. Five complementation groups have been defined for recessive mutations that confer an increase-in-ploidy (ipl) phenotype, which, in each case, cosegregates with a temperature-sensitive growth phenotype. Four new alleles of CDC31, which is required for spindle pole body duplication, were also recovered from this screen. Temperature-shift experiments with ipl1 cells show that they suffer severe nondisjunction at 37 degrees. Similar experiments with ipl2 cells show that they gain entire sets of chromosomes and become arrested as unbudded cells at 37 degrees. Molecular cloning and genetic mapping show that IPL1 is a newly identified gene, whereas IPL2 is allelic to BEM2, which is required for normal bud growth.
- Publication
Genetics, 1993, Vol 135, Issue 3, p677
- ISSN
0016-6731
- Publication type
Journal Article
- DOI
10.1093/genetics/135.3.677