We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
An endogenous hybrid mRNA encodes TWE-PRIL, a functional cell surface TWEAK-APRIL fusion protein.
- Authors
Pradet-Balade, B; Medema, J P; López-Fraga, M; Lozano, J C; Kolfschoten, G M; Picard, A; Martínez-A, C; Garcia-Sanz, J A; Hahne, M
- Abstract
TWEAK and APRIL are two recently identified tumour necrosis factor (TNF) ligand family members, implicated in angiogenesis and immune regulation, respectively. TWEAK is a transmembrane protein expressed on the cell surface, whereas APRIL acts solely as a secreted factor. In this report, using RACE, RT-PCR, cDNA library screening and an RNase protection assay, we characterize a hybrid transcript between TWEAK and APRIL mRNAs. The encoded TWE-PRIL protein is composed of TWEAK cytoplasmic and transmembrane domains fused to the APRIL C-terminal domain. TWE-PRIL mRNA is expressed and translated in human primary T cells and monocytes, and endogenous TWE-PRIL protein was detected in primary human T lymphocytes and monocytic cell lines. TWE-PRIL is membrane anchored and presents the APRIL receptor-binding domain at the cell surface. It is a biologically active ligand, as it stimulates cycling in T- and B-lymphoma cell lines. Much like membrane-bound and secreted TNF-alpha, the different cellular localizations of TWE-PRIL and APRIL suggest that they exert distinct biological roles.
- Publication
The EMBO journal, 2002, Vol 21, Issue 21, p5711
- ISSN
0261-4189
- Publication type
Journal Article
- DOI
10.1093/emboj/cdf565