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- Title
plo1<sup>+</sup> regulates gene transcription at the M-G<sub>1</sub> interval during the fission yeast mitotic cell cycle.
- Authors
Anderson, Mark; Szu Shien Ng; Marchesi, Vanessa; Maclver, Fiona H.; Stevens, Frances E.; Riddell, Tracy; Glover, David M.; Hagan, Iain M.; Mclnerny, Christopher J.
- Abstract
The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G1 interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15+ promoter that we call a PCB (pombe cell cycle box), which confers M-G1-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spol2+, cdc19+, fin1+, sid2+, ppb1+, mid1+/dmf1+ and plo1+, which we find to be transcribed at M-G1. A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB- binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Ploip protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
- Publication
EMBO Journal, 2002, Vol 21, Issue 21, p5745
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1093/emboj/cdf564